Recombinant Mouse Supt5 Protein, Myc/DDK-tagged
| Cat.No. : | Supt5-6228M |
| Product Overview : | Purified recombinant protein of mouse full-length suppressor of Ty 5 (Supt5), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
- Specification
- Gene Information
- Related Products
| Description : | Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. |
| Source : | HEK293T |
| Species : | Mouse |
| Tag : | Myc/DDK |
| Molecular Mass : | 121.1 kDa |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : | Store at -80 centigrade after receiving vials. |
| Concentration : | >50 μg/mL as determined by microplate BCA method |
| Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Gene Name : | Supt5 suppressor of Ty 5, DSIF elongation factor subunit [ Mus musculus (house mouse) ] |
| Official Symbol : | Supt5 |
| Synonyms : | Supt5; suppressor of Ty 5, DSIF elongation factor subunit; Sp; Spt5; Supt; Supt5h; AL033283; AL033321; AU019549; transcription elongation factor SPT5; DRB sensitivity-inducing factor large subunit; DSIF large subunit; suppressor of Ty 5 homolog |
| Gene ID : | 20924 |
| mRNA Refseq : | NM_013676 |
| Protein Refseq : | NP_038704 |
| UniProt ID : | O55201 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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