Recombinant Sea pansy Luciferase-Streptavidin Fusion Protein, His-tagged
| Cat.No. : | Luciferase-Streptavidin-01S |
| Product Overview : | Recombinant streptavidin-luciferase hybrid protein with a N-terminal 6x histidine tag was derived from E.coli. |
- Specification
- Gene Information
- Related Products
| Description : | Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
| Source : | E. coli |
| Species : | Sea pansy |
| Tag : | His |
| Form : | Liquid |
| Molecular Mass : | 54 kDa |
| Protein length : | 34-1116aa |
| Purity : | > 95% by SDS-PAGE under reducing conditions and visualized by Coomassie blue staining. |
| Applications : | ELISA, Dot Blotting, etc. For biotin binding affinity test, the same amount of recombinant streptavidin-luciferase fusion protein and the commercial native streptavidin was used to coat 96-well plates, respectively. After addition of the same amount of serial-diluted biotin, the bound biotin was further probed with anti-biotin antibody prior to thoroughly washing. In this assay, the minimum amount of biotin bound to recombinant streptavidin-luciferase and commercial native streptavidin was very similar. For luminescence activity, the recombinant streptavidin-luciferase produces an appropriate 1.8 X 109 RLU per microgram protein using the Renilla Lucifearse assay kit. |
| Storage : | Upon arrival, the protein may be stored for two weeks at 2 to 8 centigrade. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : | Supplied as a 0.2 μm filtered solution in PBS (pH 7.4). |
| Shipping : | ShippingIce packs |
| Official Symbol : | Luciferase-Streptavidin |
| Synonyms : | Luciferase-Streptavidin; Luciferase; Streptavidin; ec 1.13.12.7; Firefly; Luciferase; Luciferin 4 monooxygenase |
| Official Symbol : | Luciferase-Streptavidin |
| Synonyms : | Luciferase-Streptavidin; Luciferase; Streptavidin; ec 1.13.12.7; Firefly; Luciferase; Luciferin 4 monooxygenase |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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