Unactive Recombinant Human LIMK1(285-638) Protein, N-His-tagged

• Cat.No.: LIMK1-124H
• Product Overview: N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285–638, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 285-638 aa
• Form: 1.07mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: As provided, this lot demonstrated 3.8% of maximum activity. Activated by phosphorylation with ROCKII.
• Molecular Mass: 41.8 kDa
• AA Sequence: 1 MHHHHHHEFG SSARQKPVLR SCSIDRSPGA GSLGSPASQR KDLGRSESLR VVCRPHRIFR 61 PSDLIHGEVL GKGCFGQAIK VTHRETGEVM VMKELIRFDE ETQRTFLKEV KVMRCLEHPN 121 VLKFIGVLYK DKRLNFITEY IKGGTLRGII KSMDSQYPWS QRVSFAKDIA SGMAYLHSMN 181 IIHRDLNSHN CLVRENKNVV VADFGLARLM VDEKTQPEGL RSLKKPDRKK RYTVVGNPYW 241 MAPEMINGRS YDEKVDVFSF GIVLCEIIGR VNADPDYLPR TMDFGLNVRG FLDRYCPPNC 301 PPSFFPITVR CCDLDPEKRP SFVKLEHWLE TLRMHLAGHL PLGPQLEQLD RGFWETYRRG 361 ESG
• Purity: Purity 75% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ]
• Official Symbol: LIMK1
• Synonyms: LIMK1; LIM domain kinase 1; LIMK; LIMK-1
• Gene ID: 3984
• mRNA Refseq: NM_002314.4
• Protein Refseq: NP_002305.1
• MIM: 601329
• UniProt ID: P53667