Activated Recombinant Human HCK(230-497) Protein, N-His-tagged

• Cat.No.: HCK-97H
• Product Overview: N-terminal 6His-tagged, recombinant, human Hck, amino acids 230–497 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose. Auto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Specification

• Description: Hematopoietic cell kinase (HCK) is a member of Src protein-tyrosine kinase family and expressed primary in granulocytes, monocytes and macrophages. Active HCK phosphorylates STAT5B on Tyr699, which represents an essential step in Signal transducer and activator of transcription 5 (STAT5B) stimulation. The binding of SH3 ligands, such as the Nef protein of HIV-1, further augments Hck activity suggesting a new mechanism for regulating tyrosine kinase activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 230-497 aa
• Form: 0.824mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.
• Bio-activity: 40900U/mg, where one unit of Hck activity is defined as 1nmol phosphate incorporated into 250μM (GGMEDIYFEFMGGKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 34 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSEK DAWEIPRESL KLEKKLGAGQ FGEVWMATYN 61 KHTKVAVKTM KPGSMSVEAF LAEANVMKTL QHDKLVKLHA VVTKEPIYII TEFMAKGSLL 121 DFLKSDEGSK QPLPKLIDFS AQIAEGMAFI EQRNYIHRDL RAANILVSAS LVCKIADFGL 181 ARVIEDNEYT AREGAKFPIK WTAPEAINFG SFTIKSDVWS FGILLMEIVT YGRIPYPGMS 241 NPEVIRALER GYRMPRPENC PEELYNIMMR CWKNRPEERP TFEYIQSVLD DFYTAT
• Purity: Purity 80% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: HCK HCK proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: HCK
• Synonyms: HCK; HCK proto-oncogene, Src family tyrosine kinase; JTK9; AIPCV; p59Hck; p61Hck
• Gene ID: 3055
• mRNA Refseq: NM_001172129.3
• Protein Refseq: NP_001165600.1
• MIM: 142370
• UniProt ID: P08631