Active Recombinant Cynomolgus SIRPA protein, His-tagged

• Cat.No.: SIRPA-343C
• Product Overview: Recombinant Cynomolgus SIRPA protein(Glu 31 - Asn 370 ), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag at 2 μg/mL (100 μL/well) can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-1.25 μg/mL. Immobilized Biotinylated Human CD47, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2ug/well) plate, can bind Cynomolgus SIRP alpha, His Tag with a linear range of 0.039-0.625 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Glu 31 - Asn 370
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.