Active Recombinant Full Length Human CAMKK2 Protein, N-His-tagged

• Cat.No.: CAMKK2-36HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human CaMKK2 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca2+ signals. Seems to be involved in hippocampal activation of CREB1. May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.115mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 165U/mg, where one unit of CaMKK2, active activity is defined as 1nmol phosphate incorporated into 150μM substrate peptide (LSNLYHQGKFLQTFCGSPLYRRR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 64 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMSSC VSSQPSSNRA APQDELGGRG 61 SSSSESQKPC EALRGLSSLS IHLGMESFIV VTECEPGCAV DLGLARDRPL EADGQEVPLD 121 SSGSQARPHL SGRKLSLQER SQGGLAAGGS LDMNGRCICP SLPYSPVSSP QSSPRLPRRP 181 TVESHHVSIT GMQDCVQLNQ YTLKDEIGKG SYGVVKLAYN ENDNTYYAMK VLSKKKLIRQ 241 AGFPRRPPPR GTRPAPGGCI QPRGPIEQVY QEIAILKKLD HPNVVKLVEV LDDPNEDHLY 301 MVFELVNQGP VMEVPTLKPL SEDQARFYFQ DLIKGIEYLH YQKIIHRDIK PSNLLVGEDG 361 HIKIADFGVS NEFKGSDALL SNTVGTPAFM APESLSETRK IFSGKALDVW AMGVTLYCFV 421 FGQCPFMDER IMCLHSKIKS QALEFPDQPD IAEDLKDLIT RMLDKNPESR IVVPEIKLHP 481 WVTRHGAEPL PSEDENCTLV EVTEEEVENS VKHIPSLATV ILVKTMIRKR SFGNPFEGSR 541 REERSLSAPG NLLTKQGSED NLQGTDPPPV GEEEVLL
• Purity: Purity 85.9% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CAMKK2 calcium/calmodulin dependent protein kinase kinase 2 [ Homo sapiens (human) ]
• Official Symbol: CAMKK2
• Synonyms: CAMKK2; calcium/calmodulin dependent protein kinase kinase 2; CAMKK; CAMKKB
• Gene ID: 10645
• mRNA Refseq: NM_001270485.2
• Protein Refseq: NP_001257414.1
• MIM: 615002
• UniProt ID: Q96RR4