Active Recombinant Full Length Human CDKL2 Protein, N-His-tagged

• Cat.No.: CDKL2-55HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full length expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CDKL2, cyclin dependent kinase like 2, is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the cytoplasm, with lower levels in the nucleus.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 5.72 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 15 U/mg, where one unit of CDKL2 activity is defined as 1 nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 59.8 kDa
• Purity: 72% by SDS-PAGE and Coomassie blue staining
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDKL2 cyclin dependent kinase like 2 [ Homo sapiens (human) ]
• Official Symbol: CDKL2
• Synonyms: CDKL2; cyclin dependent kinase like 2; P56; KKIAMRE
• Gene ID: 8999
• mRNA Refseq: NM_003948.5
• Protein Refseq: NP_003939.1
• MIM: 603442
• UniProt ID: Q92772