Active Recombinant Full Length Human CDKL3 Protein, N-His-tagged

• Cat.No.: CDKL3-56HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full-length,human CDKL3, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CDKL3, cyclin dependent kinase like 3, is a member of cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This gene was identified as a gene absent in leukemic patients with chromosome 5q deletion. This loss may be an important determinant of dysmyelopoiesis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.31 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 4 U/mg, where one unit of CDKL3 activity is defined as 1 nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 55 kDa
• Purity: 71% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDKL3 cyclin dependent kinase like 3 [ Homo sapiens (human) ]
• Official Symbol: CDKL3
• Synonyms: CDKL3; cyclin dependent kinase like 3; NKIAMRE
• Gene ID: 51265
• mRNA Refseq: NM_001113575.2
• Protein Refseq: NP_001107047.1
• MIM: 608459
• UniProt ID: Q8IVW4