Active Recombinant Full Length Human CSK Protein, N-GST-tagged

• Cat.No.: CSK-61HFL
• Product Overview: Recombinant full-length human CSK, containing an N-terminal GST-tag incorporating a PreScission Protease cleavage site. Expressed in E.coli cells. Purified using GST agarose.

Specification

• Description: CSK (C-SRC, c-Src tyrosine kinase) is a non-receptor tyrosine kinase that catalyzes the tyrosine phosphorylation of Src family members at C-terminal, promoting an intramolecular association between the phosphotryosine and the Src homology 2 (SH2) domain and negatively regulating Src catalytic activity in vivo. Although CSK has substantial homology to Src kinases, it lacks the C-terminal regulatory tyrosine found in Src kinases.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.742mg/ml of enzyme in 50mM Tris/HCl, pH7.5, 0.1mM EGTA, 150mM NaCl, 1mM benzamidine, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol. Liquid at -20 centigrade.
• Bio-activity: 357U/mg where one unit equals the incorporation of 1nmol of phosphate per min into 0.1mg/ml poly(Glu,Tyr) (4:1), at 30 centigrade using 100μM ATP.
• Molecular Mass: 77.6 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SMSAIQAAWP 241 SGTECIAKYN FHGTAEQDLP FCKGDVLTIV AVTKDPNWYK AKNKVGREGI IPANYVQKRE 301 GVKAGTKLSL MPWFHGKITR EQAERLLYPP ETGLFLVRES TNYPGDYTLC VSCDGKVEHY 361 RIMYHASKLS IDEEVYFENL MQLVEHYTSD ADGLCTRLIK PKVMEGTVAA QDEFYRSGWA 421 LNMKELKLLQ TIGKGEFGDV MLGDYRGNKV AVKCIKNDAT AQAFLAEASV MTQLRHSNLV 481 QLLGVIVEEK GGLYIVTEYM AKGSLVDYLR SRGRSVLGGD CLLKFSLDVC EAMEYLEGNN 541 FVHRDLAARN VLVSEDNVAK VSDFGLTKEA SSTQDTGKLP VKWTAPEALR EKKFSTKSDV 601 WSFGILLWEI YSFGRVPYPR IPLKDVVPRV EKGYKMDAPD GCPPAVYEVM KNCWHLDAAM 661 RPSFLQLREQ LEHIKTHELH L
• Purity: Purity 100%.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CSK C-terminal Src kinase [ Homo sapiens (human) ]
• Official Symbol: CSK
• Synonyms: CSK; C-terminal Src kinase; MGC117393
• Gene ID: 1445
• mRNA Refseq: NM_001127190.2
• Protein Refseq: NP_001120662.1
• MIM: 124095
• UniProt ID: P41240