| Description : |
Casein Kinase 2 Alpha 2 is a catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. It phosphorylates acidic proteins such as casein, and is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythms. This heterotetrameric kinase includes two catalytic subunits, either alpha or alpha', and two regulatory beta subunits. The closely related gene paralog encoding the alpha, or alpha 1 subunit (CSNK2A1, Gene ID: 1457) is found on chromosome 20. An intronic variant in this gene (alpha 2) may be associated with leukocyte telomere length in a South Asian population. A related transcribed pseudogene is found on chromosome 11. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
1.719mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
3079U/mg, where one unit of Casein Kinase 2α2, active activity is defined as 1nmol phosphate incorporated into 165μM casein Kinase 2 substrate peptide (RRRDDDSDDD) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
45.8 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMPGP AAGSRARVYA EVNSLRSREY 61 WDYEAHVPSW GNQDDYQLVR KLGRGKYSEV FEAINITNNE RVVVKILKPV KKKKIKREVK 121 ILENLRGGTN IIKLIDTVKD PVSKTPALVF EYINNTDFKQ LYQILTDFDI RFYMYELLKA 181 LDYCHSKGIM HRDVKPHNVM IDHQQKKLRL IDWGLAEFYH PAQEYNVRVA SRYFKGPELL 241 VDYQMYDYSL DMWSLGCMLA SMIFRREPFF HGQDNYDQLV RIAKVLGTEE LYGYLKKYHI 301 DLDPHFNDIL GQHSRKRWEN FIHSENRHLV SPEALDLLDK LLRYDHQQRL TAKEAMEHPY 361 FYPVVKEQSQ PCADNAVLSS GLTAAR |
| Purity : |
Purity 93% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
