Active Recombinant Full Length Human GRK2 Protein, N-His-tagged

• Cat.No.: GRK2-72HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human GRK2 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: GRK2 phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 3.50mg/ml of enzyme in 50mM Tris/HCl pH7.5, 100mM NaCl, 0.5mM EDTA, 0.05% Triton X-100, 50% glycerol, 4mM DTT. Frozen solution.
• Bio-activity: 71U/mg, where one unit of GRK2, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 82 kDa
• AA Sequence: 1 MHHHHHHEFK GLRRQMADLE AVLADVSYLM AMEKSKATPA ARASKKILLP EPSIRSVMQK 61 YLEDRGEVTF EKIFSQKLGY LLFRDFCLNH LEEARPLVEF YEEIKKYEKL ETEEERVARS 121 REIFDSYIMK ELLACSHPFS KSATEHVQGH LGKKQVPPDL FQPYIEEICQ NLRGDVFQKF 181 IESDKFTRFC QWKNVELNIH LTMNDFSVHR IIGRGGFGEV YGCRKADTGK MYAMKCLDKK 241 RIKMKQGETL ALNERIMLSL VSTGDCPFIV CMSYAFHTPD KLSFILDLMN GGDLHYHLSQ 301 HGVFSEADMR FYAAEIILGL EHMHNRFVVY RDLKPANILL DEHGHVRISD LGLACDFSKK 361 KPHASVGTHG YMAPEVLQKG VAYDSSADWF SLGCMLFKLL RGHSPFRQHK TKDKHEIDRM 421 TLTMAVELPD SFSPELRSLL EGLLQRDVNR RLGCLGRGAQ EVKESPFFRS LDWQMVFLQK 481 YPPPLIPPRG EVNAADAFDI GSFDEEDTKG IKLLDSDQEL YRNFPLTISE RWQQEVAETV 541 FDTINAETDR LEARKKAKNK QLGHEEDYAL GKDCIMHGYM SKMGNPFLTQ WQRRYFYLFP 601 NRLEWRGEGE APQSLLTMEE IQSVEETQIK ERKCLLLKIR GGKQFILQCD SDPELVQWKK 661 ELRDAYREAQ QLVQRVPKMK NKPRSPVVEL SKVPLVQRGS ANGL
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK2 G protein-coupled receptor kinase 2 [ Homo sapiens (human) ]
• Official Symbol: GRK2
• Synonyms: GRK2; G protein-coupled receptor kinase 2; BARK1; ADRBK1; BETA-ARK1
• Gene ID: 156
• mRNA Refseq: NM_001619.5
• Protein Refseq: NP_001610.2
• MIM: 109635
• UniProt ID: P25098