Active Recombinant Full Length Human GRK3 Protein, C-His-tagged
Cat.No. : | GRK3-73HFL |
Product Overview : | C-terminal 6His-tagged, recombinant, full length, human GRK3, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. |
- Specification
- Gene Information
- Related Products
Description : | GRK3, G protein-coupled receptor kinase 3, is a beta-adrenergic receptor kinase that specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. |
Source : | Sf21 |
Species : | Human |
Tag : | His |
Protein length : | Full Length |
Form : | 3.31 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : | 54 U/mg, where one unit of GRK3 activity is defined as 1 nmol phosphate incorporated into 2 mg/ml Casein per minute at 30 centigrade with a final ATP concentration of 100 μM. |
Molecular Mass : | 82 kDa |
Purity : | 94% by SDS-PAGE and Coomassie blue staining. |
Applications : | Drug Discovery & Development |
Storage : | 1 year at -70 centigrade. |
Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
Gene Name : | GRK3 G protein-coupled receptor kinase 3 [ Homo sapiens (human) ] |
Official Symbol : | GRK3 |
Synonyms : | GRK3; G protein-coupled receptor kinase 3; BARK2; ADRBK2 |
Gene ID : | 157 |
mRNA Refseq : | NM_005160.4 |
Protein Refseq : | NP_005151.2 |
MIM : | 109636 |
UniProt ID : | P35626 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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