Recombinant Human GRK3 Protein, His-tagged

• Cat.No.: GRK3-387H
• Product Overview: Recombinant Human GRK3(Phe191~Phe453) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Phe191~Phe453
• Description: The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function.
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 33.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others

Gene Information

• Gene Name: GRK3 G protein-coupled receptor kinase 3 [ Homo sapiens ]
• Official Symbol: GRK3
• Synonyms: BARK2; ADRBK2; adrenergic, beta, receptor kinase 2; beta-ARK-2
• Gene ID: 157
• mRNA Refseq: NM_005160
• Protein Refseq: NP_005151
• UniProt ID: P35626