Active Recombinant Full Length Human MAK Protein, N-GST-tagged

• Cat.No.: MAK-97HFL
• Product Overview: N-terminal GST-tagged, recombinant, human MAK, full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose followed by size exclusion chromatography.

Specification

• Description: MAK, also called Male Germ Cell Associated Kinase, is a serine/threonine protein kinase and is involved in cell cycle regulation. It plays a role in the transcriptional coactivation of AR and may play a role in chromosomal stability in prostate cancer cells.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 0.32 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 6 U/mg, where one unit of MAK activity is defined as 1 nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 80 kDa
• Purity: 60% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAK male germ cell associated kinase [ Homo sapiens (human) ]
• Official Symbol: MAK
• Synonyms: MAK; male germ cell associated kinase; RP62
• Gene ID: 4117
• mRNA Refseq: NM_005906.6
• Protein Refseq: NP_005897.1
• MIM: 154235
• UniProt ID: P20794