Active Recombinant Full Length Human MYLK2 Protein, C-His-tagged

• Cat.No.: MYLK2-112HFL
• Product Overview: C-terminal 6Histagged, recombinant, human MYLK2 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: MYLK2 (Myosin Light Chain Kinase 2) is a serine/threonine protein kinase that belongs to the myosin light chain kinase family of kinases. It is involved in the regulation of smooth muscle contraction and cell migration. MYLK2 plays a key role in the regulation of smooth muscle contraction by phosphorylating myosin light chains, which triggers the contraction of the smooth muscle cells. In addition to its role in smooth muscle contraction, MYLK2 has been implicated in the regulation of cell migration. It has been shown to play a role in the formation of focal adhesions, which are critical for the attachment of cells to extracellular matrix and for the transmission of signals that regulate cell migration.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.24mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3716U/mg, where one unit of MYLK2, active activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 66 kDa
• AA Sequence: 1 MATENGAVEL GIQNPSTDKA PKGPTGERPL AAGKDPGPPD PKKAPDPPTL KKDAKAPASE 61 KGDGTLAQPS TSSQGPKGEG DRGGGPAEGS AGPPAALPQQ TATPETSVKK PKAEQGASGS 121 QDPGKPRVGK KAAEGQAAAR RGSPAFLHSP SCPAIISSSE KLLAKKPPSE ASELTFEGVP 181 MTHSPTDPRP AKAEEGKNIL AESQKEVGEK TPGQAGQAKM QGDTSRGIEF QAVPSEKSEV 241 GQALCLTARE EDCFQILDDC PPPPAPFPHR MVELRTGNVS SEFSMNSKEA LGGGKFGAVC 301 TCMEKATGLK LAAKVIKKQT PKDKEMVLLE IEVMNQLNHR NLIQLYAAIE TPHEIVLFME 361 YIEGGELFER IVDEDYHLTE VDTMVFVRQI CDGILFMHKM RVLHLDLKPE NILCVNTTGH 421 LVKIIDFGLA RRYNPNEKLK VNFGTPEFLS PEVVNYDQIS DKTDMWSMGV ITYMLLSGLS 481 PFLGDDDTET LNNVLSGNWY FDEETFEAVS DEAKDFVSNL IVKDQRARMN AAQCLAHPWL 541 NNLAEKAKRC NRRLKSQILL KKYLMKRRWK KNFIAVSAAN RFKKISSSGA LMALGVGPGP 601 GHHHHHH
• Purity: Purity 84% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MYLK2 myosin light chain kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MYLK2
• Synonyms: MYLK2; myosin light chain kinase 2; KMLC; MLCK; MLCK2; skMLCK
• Gene ID: 85366
• mRNA Refseq: NM_033118.4
• Protein Refseq: NP_149109.1
• MIM: 606566
• UniProt ID: Q9H1R3