Active Recombinant Full Length Human PRKX Protein, N-His-tagged

• Cat.No.: PRKX-163HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human PrKX expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: PRKX (Protein Kinase X-Linked) is regulated by and mediating cAMP signaling in cells. It acts through phosphorylation of downstream targets that may include CREB, SMAD6 and PKD1 and has multiple functions in cellular differentiation and epithelial morphogenesis. It also regulates myeloid cell differentiation through SMAD6 phosphorylation. In addition, PRKX is involved in nephrogenesis by stimulating renal epithelial cell migration and tubulogenesis, and slso involved in angiogenesis through stimulation of endothelial cell proliferation, migration and vascular-like structure formation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 3.944mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1015U/mg, where one unit of PrKX activity is defined as 1nmol phosphate incorporated into PAKtide (RRRLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 44.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEAPGLAQAA AAESDSRKVA EETPDGAPAL 61 CPSPEALSPE PPVYSLQDFD TLATVGTGTF GRVHLVKEKT AKHFFALKVM SIPDVIRLKQ 121 EQHVHNEKSV LKEVSHPFLI RLFWTWHDER FLYMLMEYVP GGELFSYLRN RGRFSSTTGL 181 FYSAEIICAI EYLHSKEIVY RDLKPENILL DRDGHIKLTD FGFAKKLVDR TWTLCGTPEY 241 LAPEVIQSKG HGRAVDWWAL GILIFEMLSG FPPFFDDNPF GIYQKILAGK IDFPRHLDFH 301 VKDLIKKLLV VDRTRRLGNM KNGANDVKHH RWFRSVDWEA VPQRKLKPPI VPKIAGDGDT 361 SNFETYPEND WDTAAPVPQK DLEIFKNF
• Purity: Purity 92% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKX protein kinase cAMP-dependent X-linked catalytic subunit [ Homo sapiens (human) ]
• Official Symbol: PRKX
• Synonyms: PRKX; protein kinase cAMP-dependent X-linked catalytic subunit; PKX1
• Gene ID: 5613
• mRNA Refseq: NM_005044.5
• Protein Refseq: NP_005035.1
• MIM: 300083
• UniProt ID: P51817