Active Recombinant Human ABL1(Y253F)(27-end) Mutation Protein, N-His-tagged

• Cat.No.: ABL1-08H
• Product Overview: N-terminal 6His-tagged recombinant human Abl, residues 27–end containing the Y253F mutation. Expressed by baculovirus in Sf21 insect cells. Purified using immobilised metal affinity chromatography.

Specification

• Description: The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukaemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Y253F mutation, which confers resistance to drug binding.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 27-end, Y253F
• Form: 1.071mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: 2351U/mg, where one unit of Abl (Y253F), active activity is defined as 1nmol phosphate incorporated into 50μM Abltide (EAIYAAPFAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 121.4 kDa
• AA Sequence: 1 MHHHHHHEFE ALQRPVASDF EPQGLSEAAR WNSKENLLAG PSENDPNLFV ALYDFVASGD 61 NTLSITKGEK LRVLGYNHNG EWCEAQTKNG QGWVPSNYIT PVNSLEKHSW YHGPVSRNAA 121 EYLLSSGING SFLVRESESS PGQRSISLRY EGRVYHYRIN TASDGKLYVS SESRFNTLAE 181 LVHHHSTVAD GLITTLHYPA PKRNKPTVYG VSPNYDKWEM ERTDITMKHK LGGGQFGEVY 241 EGVWKKYSLT VAVKTLKEDT MEVEEFLKEA AVMKEIKHPN LVQLLGVCTR EPPFYIITEF 301 MTYGNLLDYL RECNRQEVNA VVLLYMATQI SSAMEYLEKK NFIHRDLAAR NCLVGENHLV 361 KVADFGLSRL MTGDTYTAHA GAKFPIKWTA PESLAYNKFS IKSDVWAFGV LLWEIATYGM 421 SPYPGIDLSQ VYELLEKDYR MERPEGCPEK VYELMRACWQ WNPSDRPSFA EIHQAFETMF 481 QESSISDEVE KELGKQGVRG AVSTLLQAPE LPTKTRTSRR AAEHRDTTDV PEMPHSKGQG 541 ESDPLDHEPA VSPLLPRKER GPPEGGLNED ERLLPKDKKT NLFSALIKKK KKTAPTPPKR 601 SSSFREMDGQ PERRGAGEEE GRDISNGALA FTPLDTADPA KSPKPSNGAG VPNGALRESG 661 GSGFRSPHLW KKSSTLTSSR LATGEEEGGG SSSKRFLRSC SASCVPHGAK DTEWRSVTLP 721 RDLQSTGRQF DSSTFGGHKS EKPALPRKRA GENRSDQVTR GTVTPPPRLV KKNEEAADEV 781 FKDIMESSPG SSPPNLTPKP LRRQVTVAPA SGLPHKEEAG KGSALGTPAA AEPVTPTSKA 841 GSGAPGGTSK GPAEESRVRR HKHSSESPGR DKGKLSRLKP APPPPPAASA GKAGGKPSQS 901 PSQEAAGEAV LGAKTKATSL VDAVNSDAAK PSQPGEGLKK PVLPATPKPQ SAKPSGTPIS 961 PAPVPSTLPS ASSALAGDQP SSTAFIPLIS TRVSLRKTRQ PPERIASGAI TKGVVLDSTE 1021 ALCLAISRNS EQMASHSAVL EAGKNLYTFC VSYVDSIQQM RNKFAFREAI NKLENNLREL 1081 QICPATAGSG PAATQDFSKL LSSVKEISDI VQR
• Purity: Purity 62% by SDS-PAGE and Coomassie staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
• References: Shah et al., (2002), Cancer Cell 2, 117-125.

Gene Information

• Gene Name: ABL1 ABL proto-oncogene 1, non-receptor tyrosine kinase [ Homo sapiens (human) ] Gene ID: 25, updated on 15-Oct-2023 Download Datasets
• Official Symbol: ABL1
• Synonyms: ABL1; ABL proto-oncogene 1, non-receptor tyrosine kinase; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; BCR-ABL; bcr/abl
• Gene ID: 25
• mRNA Refseq: NM_005157.6
• Protein Refseq: NP_005148.2
• MIM: 189980
• UniProt ID: P00519