Active Recombinant Human ARAF(Y301D, Y302D)(273-end) Mutation Protein, N-GST-tagged

• Cat.No.: ARAF-11H
• Product Overview: N-terminal GSTtagged, recombinant, human A-Raf, amino acids 273-end, containing the mutations Y301D and Y302D, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of cellular protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 273-end
• Form: 0.14mg/ml of enzyme in 40mM Tris/HCl pH8.0, 200mM NaCl, 0.08mM EGTA, 0.024% Brij-35, 4mM glutathione, 20% (v/v) glycerol, 0.8mM benzamidine, 0.16mM PMSF, 0.08% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 749U/mg, where one unit of A-Raf, active activity is defined as 1nmol phosphate incorporated into 0.5mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 65 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KSPAEQRERK 241 SLADDKKKVK NLGYRDSGDD WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVSQ 301 PTAEQAQAFK NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD 361 MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT VKTRWSGAQP 421 LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL MTGSLPYSHI GCRDQIIFMV 481 GRGYLSPDLS KISSNCPKAM RRLLSDCLKF QREERPLFPQ ILATIELLQR SLPKIERSAS 541 EPSLHRTQAD ELPACLLSAA RLVP
• Purity: Purity 58% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ARAF A-Raf proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: ARAF
• Synonyms: ARAF; A-Raf proto-oncogene, serine/threonine kinase; PKS2; A-RAF; ARAF1; RAFA1
• Gene ID: 369
• mRNA Refseq: NM_001654.5
• Protein Refseq: NP_001645.1
• MIM: 311010
• UniProt ID: P10398