Active Recombinant Human CDKL4(2-end) Protein, N-His-tagged

• Cat.No.: CDKL4-45H
• Product Overview: N-terminal 6His-tagged, recombinant, human CDKL4 amino acids 2-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CDKL4, cyclin dependent kinase like 4, is predicted to enable cyclin-dependent protein serine/threonine kinase activity and to be involved in protein phosphorylation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 1.32 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 16 U/mg, where one unit of CDKL4 activity is defined as 1 nmol phosphate incorporated into 250 μM RRRFRPASPLRGPPK per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 40 kDa
• Purity: 26% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CDKL4 cyclin dependent kinase like 4 [ Homo sapiens (human) ]
• Official Symbol: CDKL4
• Synonyms: CDKL4; cyclin dependent kinase like 4; Cyclin Dependent Kinase Like 4; EC 2.7.11.2; EC 2.7.11
• Gene ID: 344387
• mRNA Refseq: NM_001009565.2
• Protein Refseq: NP_001009565.1
• UniProt ID: Q5MAI5