| Description : |
DCAMKL3 (Doublecortin and CaM Kinase-Like 3), also known as microtubule affinity-regulating kinase 3 (MARK3), is a serine/threonine protein kinase that belongs to the CAMK (calcium/calmodulin-dependent protein kinase) family of kinases. It is involved in a variety of cellular processes, including regulation of microtubule dynamics, cell polarity, and cell migration. Research has suggested that DCAMKL3 plays a role in several diseases, including cancer and Alzheimer's disease. In cancer, DCAMKL3 has been shown to promote tumor growth and metastasis, and inhibition of DCAMKL3 has been suggested as a potential cancer therapy. In Alzheimer's disease, DCAMKL3 has been implicated in the regulation of tau phosphorylation and aggregation, and alterations in DCAMKL3 expression have been observed in the brains of Alzheimer's disease patients. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
345-end |
| Form : |
0.365mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
2527U/mg, where one unit of DCAMKL3, active activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
38 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MGIIAANVEK HYETGRVIGD GNFAVVKECR 61 HRETRQAYAM KIIDKSRLKG KEDMVDSEIL IIQSLSHPNI VKLHEVYETD MEIYLILEYV 121 QGGDLFDAII ESVKFPEPDA ALMIMDLCKA LVHMHDKSIV HRDLKPENLL VQRNEDKSTT 181 LKLADFGLAK HVVRPIFTVC GTPTYVAPEI LSEKGYGLEV DMWAAGVILY ILLCGFPPFR 241 SPERDQDELF NIIQLGHFEF LPPYWDNISD AAKDLVSRLL VVDPKKRYTA HQVLQHPWIE 301 TAGKTNTVKR QKQVSPSSEG HFRSQHKRVV EQVS |
| Purity : |
Purity 97% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
