Active Recombinant Human EPHB3(599-920) Protein, N-His-tagged

• Cat.No.: EPHB3-74H
• Product Overview: N-terminal 6Histagged recombinant human EphB3 residues 599–920, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: Eph family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EphA4 can cross-respond to both ephrin-A and -B. EphB3 (Ephrin type-B receptor 3, ETK2, HEK2, TYRO6) has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 599-920 aa
• Form: 4.7mg/ml of enzyme in 50mM Tris/HCl pH7.5, 1mM EGTA, 150mM NaCl, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethnaol. Frozen solution.
• Bio-activity: 29U/mg, where one unit of EphB3 activity is defined as 1nmol phosphate incorporated Into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 40 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSQY IAPGMKVYID PFTYEDPNEA VREFAKEIDV 61 SCVKIEEVIG AGEFGEVCRG RLKQPGRREV FVAIKTLKVG YTERQRRDFL SEASIMGQFD 121 HPNIIRLEGV VTKSRPVMIL TEFMENCALD SFLRLNDGQF TVIQLVGMLR GIAAGMKYLS 181 EMNYVHRDLA ARNILVNSNL VCKVSDFGLS RFLEDDPSDP TYTSSLGGKI PIRWTAPEAI 241 AYRKFTSASD VWSYGIVMWE VMSYGERPYW DMSNQDVINA VEQDYRLPPP MDCPTALHQL 301 MLDCWVRDRN LRPKFSQIVN TLDKLIRNAA SLKVIASAQS GMSQPLLDRT
• Purity: Purity 93% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EPHB3 EPH receptor B3 [ Homo sapiens (human) ]
• Official Symbol: EPHB3
• Synonyms: EPHB3; EPH receptor B3; EK2; ETK2; HEK2; TYRO6
• Gene ID: 2049
• mRNA Refseq: NM_004443.4
• Protein Refseq: NP_004434.2
• MIM: 601839
• UniProt ID: P54753