| Description : |
IRE1 (Inositol-requiring enzyme 1) is a transmembrane protein kinase that is involved in the endoplasmic reticulum (ER) stress response pathway. It is a key regulator of the unfolded protein response (UPR), which is activated in response to ER stress to maintain ER homeostasis and promote cell survival. Research has suggested that IRE1 plays a role in several diseases, including cancer, diabetes, and neurodegenerative diseases. In cancer, activation of IRE1 has been shown to promote cell survival and tumor growth, and inhibition of IRE1 has been suggested as a potential cancer therapy. In diabetes, activation of IRE1 has been implicated in the development of insulin resistance and beta cell dysfunction. Additionally, IRE1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
465-end |
| Form : |
1.478mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
393U/mg, where one unit of IRE1 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml MBP per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
62 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF PLSMHQQQQL QHQQFQKELE KIQLLQQQQQ 61 QLPFHPPGDT AQDGELLDTS GPYSESSGTS SPSTSPRASN HSLCSGSSAS KAGSSPSLEQ 121 DDGDEETSVV IVGKISFCPK DVLGHGAEGT IVYRGMFDNR DVAVKRILPE CFSFADREVQ 181 LLRESDEHPN VIRYFCTEKD RQFQYIAIEL CAATLQEYVE QKDFAHLGLE PITLLQQTTS 241 GLAHLHSLNI VHRDLKPHNI LISMPNAHGK IKAMISDFGL CKKLAVGRHS FSRRSGVPGT 301 EGWIAPEMLS EDCKENPTYT VDIFSAGCVF YYVISEGSHP FGKSLQRQAN ILLGACSLDC 361 LHPEKHEDVI ARELIEKMIA MDPQKRPSAK HVLKHPFFWS LEKQLQFFQD VSDRIEKESL 421 DGPIVKQLER GGRAVVKMDW RENITVPLQT DLRKFRTYKG GSVRDLLRAM RNKKHHYREL 481 PAEVRETLGS LPDDFVCYFT SRFPHLLAHT YRAMELCSHE RLFQPYYFHE PPEPQPPVTP 541 DAL |
| Purity : |
Purity 93.2% by SDS-PAGE and Coomassie blue staining |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
