Active Recombinant Human MAPKAPK3(2-end) Protein, N-GST-tagged

• Cat.No.: MAPKAPK3-133H
• Product Overview: N-terminal GST-tagged, recombinant, human MAPKAP Kinase 3 amino acids 2–end, expressed in E.coli cells. Purified using glutathione agarose. Activated using SAPK2a (cat# 14-587) and repurified using glutathione agarose.

Specification

• Description: Mitogen-activated protein kinase (MAPK)-activated protein kinase 3 (MAPKAPK3) mediates multiple p38 MAPK-dependent inflammatory responses. This kinase can be activated in response to cellular stress and pro-inflammatory cytokines through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. p38 MAP kinase, activated through MKK3, is required for the production of inflammatory cytokines by both antigen-presenting cells and CD4+ T cells.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 2-end
• Form: 1.904mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mMbenzamidine, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 3540U/mg, where one unit of MAPKAP Kinase 3, active activity is defined as 1nmol phosphate incorporated into 30μM (KKLNRTLSVA) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 69.8 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SDGETAEEQG 241 GPVPPPVAPG GPGLGGAPGG RREPKKYAVT DDYQLSKQVL GLGVNGKVLE CFHRRTGQKC 301 ALKLLYDSPK ARQEVDHHWQ ASGGPHIVCI LDVYENMHHG KRCLLIIMEC MEGGELFSRI 361 QERGDQAFTE REAAEIMRDI GTAIQFLHSH NIAHRDVKPE NLLYTSKEKD AVLKLTDFGF 421 AKETTQNALQ TPCYTPYYVA PEVLGPEKYD KSCDMWSLGV IMYILLCGFP PFYSNTGQAI 481 SPGMKRRIRL GQYGFPNPEW SEVSEDAKQL IRLLLKTDPT ERLTITQFMN HPWINQSMVV 541 PQTPLHTARV LQEDKDHWDE VKEEMTSALA TMRVDYDQVK IKDLKTSNNR LLNKRRKKQA 601 GSSSASQGCN NQ
• Purity: Purity 97.2% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAPKAPK3 MAPK activated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK3
• Synonyms: MAPKAPK3; MAPK activated protein kinase 3; 3PK; MK3; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
• Gene ID: 7867
• mRNA Refseq: NM_004635.5
• Protein Refseq: NP_004626.1
• MIM: 602130
• UniProt ID: Q16644