Active Recombinant Human MUSK(530-end) Protein, N-His-tagged

• Cat.No.: MUSK-168H
• Product Overview: N-terminal 6Histagged, recombinant, human MuSK, amino acids 530–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: MuSK (Muscle Associated Receptor Tyrosine Kinase) is a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 530-end
• Form: 1.09mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1 % 2-mercaptoethanol. Frozen solution.
• Bio-activity: 78U/mg, where one unit of MuSK, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRLESAA VTLTTLPSEL LLDRLHPNPM 61 YQRMPLLLNP KLLSLEYPRN NIEYVRDIGE GAFGRVFQAR APGLLPYEPF TMVAVKMLKE 121 EASADMQADF QREAALMAEF DNPNIVKLLG VCAVGKPMCL LFEYMAYGDL NEFLRSMSPH 181 TVCSLSHSDL SMRAQVSSPG PPPLSCAEQL CIARQVAAGM AYLSERKFVH RDLATRNCLV 241 GENMVVKIAD FGLSRNIYSA DYYKANENDA IPIRWMPPES IFYNRYTTES DVWAYGVVLW 301 EIFSYGLQPY YGMAHEEVIY YVRDGNILSC PENCPVELYN LMRLCWSKLP ADRPSFTSIH 361 RILERMCERA EGTVSV
• Purity: Purity 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MUSK muscle associated receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: MUSK
• Synonyms: MUSK; muscle associated receptor tyrosine kinase; CMS9; FADS; FADS1
• Gene ID: 4593
• mRNA Refseq: NM_005592.4
• Protein Refseq: NP_005583.1
• MIM: 601296
• UniProt ID: O15146