Active Recombinant Human NLK(121-end) Protein, N-His-tagged

• Cat.No.: NLK-176H
• Product Overview: N-terminal 6Histagged, recombinant, human NLK, amino acids 121–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: NLK (Nemo Like Kinase) regulates a number of transcription factors with key roles in cell fate determination. It enables ubiquitin protein ligase binding activity and Involved in protein stabilization and transforming growth factor beta receptor signaling pathway. It predicted to be located in cytosol and nucleoplasm, and to be active in cytoplasm and nucleus.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 121-end
• Form: 0.54mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 17U/mg, where one unit of NLK, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF QQQLDIEPDR PIGYGAFGVV WSVTDPRDGK 61 RVALKKMPNV FQNLVSCKRV FRELKMLCFF KHDNVLSALD ILQPPHIDYF EEIYVVTELM 121 QSDLHKIIVS PQPLSSDHVK VFLYQILRGL KYLHSAGILH RDIKPGNLLV NSNCVLKICD 181 FGLARVEELD ESRHMTQEVV TQYYRAPEIL MGSRHYSNAI DIWSVGCIFA ELLGRRILFQ 241 AQSPIQQLDL ITDLLGTPSL EAMRTACEGA KAHILRGPHK QPSLPVLYTL SSQATHEAVH 301 LLCRMLVFDP SKRISAKDAL AHPYLDEGRL RYHTCMCKCC FSTSTGRVYT SDFEPVTNPK 361 FDDTFEKNLS SVRQVKEIIH QFILEQQKGN RVPLCINPQS AAFKSFISST VAQPSEMPPS 421 PLVWE
• Purity: Purity 67% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NLK nemo like kinase [ Homo sapiens (human) ]
• Official Symbol: NLK
• Synonyms: NLK; nemo like kinase; DKFZp761G1211; FLJ21033; LAK1
• Gene ID: 51701
• mRNA Refseq: NM_016231.5
• Protein Refseq: NP_057315.3
• MIM: 609476
• UniProt ID: Q9UBE8