| Description : |
Receptors that are tyrosine protein kinases include those receptors for platelet-derived growth factor (PDGF), insulin, epidermal growth factor (EGF) and certain lymphokines. Receptor tyrosine kinases are cell surface transmembrane proteins responsible for intracellular signal transduction. They are expressed in several cell types and, after activation by growth factor binding, trigger a series of intracellular pathways leading to a wide variety of responses; e.g. differentiation, proliferation, migration and invasion, angiogenesis, survival. Inhibition of PDGFA, Alpha platelet-derived growth factor receptor, PDGF-R-alpha, CD140a antigen) receptor kinase may be useful in the treatment of several tumors. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
550-end |
| Form : |
1.127mg/ml of enzyme in 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
29U/mg, where one unit of PDGFR alpha, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
63.5 kDa |
| AA Sequence : |
1 MAHHHHHHEN LYFQGKQKPR YEIRWRVIES ISPDGHEYIY VDPMQLPYDS RWEFPRDGLV 61 LGRVLGSGAF GKVVEGTAYG LSRSQPVMKV AVKMLKPTAR SSEKQALMSE LKIMTHLGPH 121 LNIVNLLGAC TKSGPIYIIT EYCFYGDLVN YLHKNRDSFL SHHPEKPKKE LDIFGLNPAD 181 ESTRSYVILS FENNGDYMDM KQADTTQYVP MLERKEVSKY SDIQRSLYDR PASYKKKSML 241 DSEVKNLLSD DNSEGLTLLD LLSFTYQVAR GMEFLASKNC VHRDLAARNV LLAQGKIVKI 301 CDFGLARDIM HDSNYVSKGS TFLPVKWMAP ESIFDNLYTT LSDVWSYGIL LWEIFSLGGT 361 PYPGMMVDST FYNKIKSGYR MAKPDHATSE VYEIMVKCWN SEPEKRPSFY HLSEIVENLL 421 PGQYKKSYEK IHLDFLKSDH PAVARMRVDS DNAYIGVTYK NEEDKLKDWE GGLDEQRLSA 481 DSGYIIPLPD IDPVPEEEDL GKRNRHSSQT SEESAIETGS SSSTFIKRED ETIEDIDMMD 541 DIGIDSSDLV EDSFL |
| Purity : |
Purity 80.3% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
