| Description : |
Receptors that are tyrosine protein kinases include those receptors for platelet-derived growth factor (PDGF), insulin, epidermal growth factor (EGF) and certain lymphokines. Receptor tyrosine kinases are cell surface transmembrane proteins responsible for intracellular signal transduction. They are expressed in several cell types and, after activation by growth factor binding, trigger a series of intracellular pathways leading to a wide variety of responses; e.g. differentiation, proliferation, migration and invasion, angiogenesis, survival. Inhibition of PDGFRB (PDGFR1, JTK12, CD140B,Beta platelet-derived growth factor) receptor kinase may be useful in the treatment of several tumors. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
557-end |
| Form : |
1.975mg/ml of enzyme in 50mM Tris/HCl pH7.5, 0.1mM EGTA, 150mM NaCl, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
64U/mg, where one unit of PDGFR beta activity is defined as 1nmol phosphate incorporated into poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
64 kDa |
| AA Sequence : |
1 MAHHHHHHEN LYFQGQKKPR YEIRWKVIES VSSDGHEYIY VDPMQLPYDS TWELPRDQLV 61 LGRTLGSGAF GQVVEATAHG LSHSQATMKV AVKMLKSTAR SSEKQALMSE LKIMSHLGPH 121 LNVVNLLGAC TKGGPIYIIT EYCRYGDLVD YLHRNKHTFL QHHSDKRRPP SAELYSNALP 181 VGLPLPSHVS LTGESDGGYM DMSKDESVDY VPMLDMKGDV KYADIESSNY MAPYDNYVPS 241 APERTCRATL INESPVLSYM DLVGFSYQVA NGMEFLASKN CVHRDLAARN VLICEGKLVK 301 ICDFGLARDI MRDSNYISKG STFLPLKWMA PESIFNSLYT TLSDVWSFGI LLWEIFTLGG 361 TPYPELPMNE QFYNAIKRGY RMAQPAHASD EIYEIMQKCW EEKFEIRPPF SQLVLLLERL 421 LGEGYKKKYQ QVDEEFLRSD HPAILRSQAR LPGFHGLRSP LDTSSVLYTA VQPNEGDNDY 481 IIPLPDPKPE VADEGPLEGS PSLASSTLNE VNTSSTISCD SPLEPQDEPE PEPQLELQVE 541 PEPELEQLPD SGCPAPRAEA EDSFL |
| Purity : |
Purity 87.3% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
