Active Recombinant Human PRKCD(2-end) Protein, N-His-tagged

• Cat.No.: PRKCD-198H
• Product Overview: N-terminal 6Histagged, recombinant amino acids 2–end of human PKC delta expressed in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Protein kinase Cd (PKCd) is one of the major protein kinase C isoforms of a family of serine/threonine protein kinases. Breakdown of phosphatidylinositol leads to formation of diacylglycerol and inositol triphosphate, the former acting synergistically with Ca2+ to activate protein kinase C (PKC), resulting in translocation of the enzyme from cytosol to the membrane. Phosphorylation of proteins by PKC has been implicated as a pivotal regulatory element in signal transduction, cellular regulation, and with a high affinity to phorbol ester tumor promoters.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.216mg/ml of enzyme in 50mM Tris/HCl pH7.5, 270mM sucrose, 150mM NaCl, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: 1280U/mg, where one unit of PKC delta, active activity is defined as 1nmol phosphate incorporated into 50μM PKCtide (ERMRPRKRQGSVRRRV) in the presence of lipid activator per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 78.7 kDa
• AA Sequence: 1 MHHHHHHEFA PFLRIAFNSY ELGSLQAEDE ANQPFCAVKM KEALSTERGK TLVQKKPTMY 61 PEWKSTFDAH IYEGRVIQIV LMRAAEEPVS EVTVGVSVLA ERCKKNNGKA EFWLDLQPQA 121 KVLMSVQYFL EDVDCKQSMR SEDEAKFPTM NRRGAIKQAK IHYIKNHEFI ATFFGQPTFC 181 SVCKDFVWGL NKQGYKCRQC NAAIHKKCID KIIGRCTGTA ANSRDTIFQK ERFNIDMPHR 241 FKVHNYMSPT FCDHCGSLLW GLVKQGLKCE DCGMNVHHKC REKVANLCGI NQKLLAEALN 301 QVTQRASRRS DSASSEPVGI YQGFEKKTGV AGEDMQDNSG TYGKIWEGSS KCNINNFIFH 361 KVLGKGSFGK VLLGELKGRG EYFAIKALKK DVVLIDDDVE CTMVEKRVLT LAAENPFLTH 421 LICTFQTKDH LFFVMEFLNG GDLMYHIQDK GRFELYRATF YAAEIMCGLQ FLHSKGIIYR 481 DLKLDNVLLD RDGHIKIADF GMCKENIFGE SRASTFCGTP DYIAPEILQG LKYTFSVDWW 541 SFGVLLYEML IGQSPFHGDD EDELFESIRV DTPHYPRWIT KESKDILEKL FEREPTKRLG 601 VTGNIKIHPF FKTINWTLLE KRRLEPPFRP KVKSPRDYSN FDQEFLNEKA RLSYSDKNLI 661 DSMDQSAFAG FSFVNPKFEH LLED
• Purity: Purity 98% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PRKCD protein kinase C delta [ Homo sapiens (human) ]
• Official Symbol: PRKCD
• Synonyms: PRKCD; protein kinase C delta; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
• Gene ID: 5580
• mRNA Refseq: NM_212539.2
• Protein Refseq: NP_997704.1
• MIM: 176977
• UniProt ID: Q05655