Active Recombinant Human PTPRM(879-1184) Protein, N-GST-tagged

• Cat.No.: PTPRM-219H
• Product Overview: N-terminal GSTtagged, recombinant, human RPTPmu, amino acids 879–1184, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: RPTPμ is a receptor protein-tyrosine phosphatase that mediates homotypic cell-cell interactions and plays a role in adipogenic differentiation via modulation of p120 catenin/CTNND1 phosphorylation. Promotes CTNND1 dephosphorylation and prevents its cytoplasmic localization where it inhibits SLC2A4 membrane trafficking. In turn, SLC2A4 is directed to the plasma membrane and performs its glucose transporter function.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 879-1184 aa
• Form: 0.93mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 32412 U/mg, where one unit of RPTPmu, active activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8- difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 61.9 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFA IRVADLLQHI 241 TQMKCAEGYG FKEEYESFFE GQSAPWDSAK KDENRMKNRY GNIIAYDHSR VRLQTIEGDT 301 NSDYINGNYI DGYHRPNHYI ATQGPMQETI YDFWRMVWHE NTASIIMVTN LVEVGRVKCC 361 KYWPDDTEIY KDIKVTLIET ELLAEYVIRT FAVEKRGVHE IREIRQFHFT GWPDHGVPYH 421 ATGLLGFVRQ VKSKSPPSAG PLVVHCSAGA GRTGCFIVID IMLDMAEREG VVDIYNCVRE 481 LRSRRVNMVQ TEEQYVFIHD AILEACLCGD TSVPASQVRS LYYDMNKLDP QTNSS
• Purity: Purity 95.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTPRM protein tyrosine phosphatase receptor type M [ Homo sapiens (human) ]
• Official Symbol: PTPRM
• Synonyms: PTPRM; protein tyrosine phosphatase receptor type M; RPTPM; RPTPU; PTPRL1; hR-PTPu; R-PTP-MU
• Gene ID: 5797
• mRNA Refseq: NM_002845.4
• Protein Refseq: NP_002836.3
• MIM: 176888
• UniProt ID: P28827