Active Recombinant Human RIPK2(1-299) Protein, N-His-tagged

• Cat.No.: RIPK2-214H
• Product Overview: N-terminal 6Histagged, recombinant, human RIPK2, amino acids 1–299, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: RIPK2 (receptor-interacting serine-threonine kinase 2, RIP2, RICK, CARD3, CARDIAK) is a death domain-containing serine-threonine kinase associated with TNF receptor and implicated in the activation of NF-kappaB and cell death in mammalian cells. RIPK2 represents a kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of pro-inflammatory cytokine IL-1β.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-299 aa
• Form: 1.097mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 54U/mg, where one unit of RIPK2 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 37.8 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSMN GEAICSALPT IPYHKLADLR YLSRGASGTV 61 SSARHADWRV QVAVKHLHIH TPLLDSERKD VLREAEILHK ARFSYILPIL GICNEPEFLG 121 IVTEYMPNGS LNELLHRKTE YPDVAWPLRF RILHEIALGV NYLHNMTPPL LHHDLKTQNI 181 LLDNEFHVKI ADFGLSKWRM MSLSQSRSSK SAPEGGTIIY MPPENYEPGQ KSRASIKHDI 241 YSYAVITWEV LSRKQPFEDV TNPLQIMYSV SQGHRPVINE ESLPYDIPHR ARMISLIESG 301 WAQNPDERPS FLKCLIELEP VLRTFEE
• Purity: Purity 76.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RIPK2 receptor interacting serine/threonine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: RIPK2
• Synonyms: RIPK2; receptor interacting serine/threonine kinase 2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK
• Gene ID: 8767
• mRNA Refseq: NM_003821.6
• Protein Refseq: NP_003812.1
• MIM: 603455
• UniProt ID: O43353