Active Recombinant Human TARDBP protein, His-tagged

• Cat.No.: TARDBP-0740H
• Product Overview: Recombinant Human TARDBP protein(Met1-Met414), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 500 mM NaCl, 0.4 M Arginine, pH8.0 with glycerol as protectant.
• Bio-activity: Immobilized Human TDP-43, His Tag at 1 μg/mL (100 μL/well) can bind Anti-TDP43 Antibody with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 46.8 kDa. The protein migrates as 54-56 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Met1-Met414
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TARDBP TAR DNA binding protein [ Homo sapiens ]
• Official Symbol: TARDBP
• Synonyms: TARDBP; TAR DNA binding protein; TAR DNA-binding protein 43; ALS10; TDP 43; TAR DNA-binding protein-43; TDP-43
• Gene ID: 23435
• mRNA Refseq: NM_007375
• Protein Refseq: NP_031401
• MIM: 605078
• UniProt ID: Q13148