Active Recombinant Human TRPM7(1180-end) Protein, N-His-tagged

• Cat.No.: TRPM7-46H
• Product Overview: N-terminal 6Histagged, recombinant, human ChaK1 amino acids 1180-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CHAK1 is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1180-end
• Form: 0.24mg/ml of enzyme in 50mM Tris/HCl pH7.5, 180mM NaCl, 0.09mM EGTA, 270mM sucrose, 259.5mM imidazole, 0.86mM benzamidine, 0.17mM PMSF, 0.09% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 1895U/mg, where one unit of ChaK1, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml MBP per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 82 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMYFN EKDDKFHSGS EERIRVTFER 61 VEQMCIQIKE VGDRVNYIKR SLQSLDSQIG HLQDLSALTV DTLKTLTAQK ASEASKVHNE 121 ITRELSISKH LAQNLIDDGP VRPSVWKKHG VVNTLSSSLP QGDLESNNPF HCNILMKDDK 181 DPQCNIFGQD LPAVPQRKEF NFPEAGSSSG ALFPSAVSPP ELRQRLHGVE LLKIFNKNQK 241 LGSSSTSIPH LSSPPTKFFV STPSQPSCKS HLETGTKDQE TVCSKATEGD NTEFGAFVGH 301 RDSMDLQRFK ETSNKIKILS NNNTSENTLK RVSSLAGFTD CHRTSIPVHS KQAEKISRRP 361 STEDTHEVDS KAALIPDWLQ DRPSNREMPS EEGTLNGLTS PFKPAMDTNY YYSAVERNNL 421 MRLSQSIPFT PVPPRGEPVT VYRLEESSPN ILNNSMSSWS QLGLCAKIEF LSKEEMGGGL 481 RRAVKVQCTW SEHDILKSGH LYIIKSFLPE VVNTWSSIYK EDTVLHLCLR EIQQQRAAQK 541 LTFAFNQMKP KSIPYSPRFL EVFLLYCHSA GQWFAVEECM TGEFRKYNNN NGDEIIPTNT 601 LEEIMLAFSH WTYEYTRGEL LVLDLQGVGE NLTDPSVIKA EEKRSCDMVF GPANLGEDAI 661 KNFRAKHHCN SCCRKLKLPD LKRNDYTPDK IIFPQDEPSD LNLQPGNSTK ESESTNSVRL 721 ML
• Purity: Purity 95% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: TRPM7 transient receptor potential cation channel subfamily M member 7 [ Homo sapiens (human) ]
• Official Symbol: TRPM7
• Synonyms: TRPM7; transient receptor potential cation channel subfamily M member 7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
• Gene ID: 54822
• mRNA Refseq: NM_017672.6
• Protein Refseq: NP_060142.3
• MIM: 605692
• UniProt ID: Q96QT4