Active Recombinant Human WNK2(166-489) Protein, N-His-tagged

• Cat.No.: WNK2-259H
• Product Overview: N-terminal 6Histagged, recombinant, human WNK2, amino acids 166–489, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: WNK2 (WNK Lysine Deficient Protein Kinase 2) is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. WNK2 plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 166-489 aa
• Form: 2.16mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 37U/mg, where one unit of WNK2, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 41.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GRTRRDEPEE EEDDEDDLKA VATSLDGRFL 61 KFDIELGRGS FKTVYKGLDT ETWVEVAWCE LQDRKLTKLE RQRFKEEAEM LKGLQHPNIV 121 RFYDFWESSA KGKRCIVLVT ELMTSGTLKT YLKRFKVMKP KVLRSWCRQI LKGLLFLHTR 181 TPPIIHRDLK CDNIFITGPT GSVKIGDLGL ATLKRASFAK SVIGTPEFMA PEMYEEHYDE 241 SVDVYAFGMC MLEMATSEYP YSECQNAAQI YRKVTCGIKP ASFEKVHDPE IKEIIGECIC 301 KNKEERYEIK DLLSHAFFAE DTGVRVELAE EDHGRKSTIA LRLWVEDPKK LKGK
• Purity: Purity 84% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: WNK2 WNK lysine deficient protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: WNK2
• Synonyms: WNK2; WNK lysine deficient protein kinase 2; PRKWNK2; NY-CO-43; SDCCAG43; P/OKcl.13
• Gene ID: 65268
• mRNA Refseq: NM_006648.4
• Protein Refseq: NP_006639.3
• MIM: 606249
• UniProt ID: Q9Y3S1