Active Recombinant Human VEGFA, Met-tagged

• Cat.No.: VEGFA-710H
• Product Overview: Recombinant Human VEGFA (Accession # NP_001191313) Ala27-Arg171, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met-Ala27 & Pro28
• Form: Lyophilized from a 0.2 µm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323.The ED50 for this effect is typically 12-60 ng/mL.
• Molecular Mass: Recombinant Human VEGFA, Met-tagged has a calculated MW of 17 kDa (monomer). In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13