Active Recombinant Human VEGFA Protein (Ala1-Arg121), C-His tagged, Animal-free, Carrier-free

• Cat.No.: VEGFA-291H
• Product Overview: Recombinant Human VEGFA Protein (Ala1-Arg121) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Vascular Endothelial Growth Factors 121(VEGF121) is one of five VEGF splice variants of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF121 is the only VEGF which lacks heparin-binding activity and freely diffusible. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1 /KDR) on endothelial cells to activate signal transduction and regulate both physiological and pathological angiogenesis.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in HUVEC cells. The ED50 for this effect is < 2.5 ng/mL.
• Molecular Mass: The protein has a calculated MW of 15.00 kDa.
  The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala1-Arg121
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens (human) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A
• Gene ID: 7422
• mRNA Refseq: NM_001171626
• Protein Refseq: NP_001165097
• MIM: 192240
• UniProt ID: A0A0Y0IMM4