||Recombinant human PSMB9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||PSMB9, also known as proteasome subunit beta type-9, is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This protein is located in the class II region of the MHC (major histocompatibility complex). Expression of this protein is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 50mM NaCl
||23.5 kDa (220aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MTTIMAVEFD GGVVMGSDSR VSAGEAVVNR VFDKLSPLHE RIYCALSGSAADAQAVADMA AYQLELHGIELEEPPLVLAA ANVVRNISYK YREDLSAHLM VAGWDQREGG QVYGTLGGML TRQPFAIGGS GSTFIYGYVD AAYKPGMSPE ECRRFTTDAIALAMSRDGSS GGVIYLVTIT AAGVDHRVIL GNELPKFYDE
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.