Active Recombinant Human FCGR2A protein, His-tagged
Cat.No. : | FCGR2A-1134H |
Product Overview : | Recombinant Human FCGR2A protein(P12318-1)(Met1-Ile218,167Arg), fused with His tag, was expressed in HEK293. |
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- Gene Information
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | 1. Measured by its ability to bind biotinylated human IgG2 in functional ELISA.2.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRIIA / CD32a (R167) recombinant protein with an affinity constant of 2.3 μM as determined in a BLI assay (ForteBio Octet Red384).3.Loaded Human FcγRIIA / CD32a (R167) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.65 μM as determined in a BLI assay (ForteBio Octet Red384).4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIA / CD32a (R167) recombinant protein with an affinity constant of 0.79 µM as determined in an SPR assay (Biacore T200). |
Molecular Mass : | The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. |
Proteinlength : | Met1-Ile218,167Arg |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Publication : |
The Characterisation of FcγRIIa Antagonists (2016)
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Gene Name : | FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ] |
Official Symbol : | FCGR2A |
Synonyms : | FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032; |
Gene ID : | 2212 |
mRNA Refseq : | NM_001136219 |
Protein Refseq : | NP_001129691 |
MIM : | 146790 |
UniProt ID : | P12318 |
Products Types
◆ Recombinant Protein | ||
FCGR2A-1962R | Recombinant Rat FCGR2A Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGR2A-543H | Recombinant Human FCGR2A Protein (H167) | +Inquiry |
FCGR2A-3990H | Recombinant Human FCGR2A Protein | +Inquiry |
FCGR2A-264C | Recombinant Cynomolgus Monkey FCGR2A Protein, His (Fc)-Avi-tagged | +Inquiry |
FCGR2A-384H | Active Recombinant Human FCGR2A Protein (R167) (Ala 36 - Ile 218), His & Avi-tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
FCGR2A-1996HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
FCGR2A-001HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
FCGR2A-1926CCL | Recombinant Cynomolgus FCGR2A cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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