Active Recombinant Murine Lep protein

• Cat.No.: Lep-337M
• Product Overview: Recombinant Murine Lep was expressed in E. coli.

Specification

• Description: Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.
• Source: E. coli
• Species: Murine
• Form: Sterile filtered through a 0.2 micron filter. Lyophilized with 0.1% TFA.
• Bio-activity: Creative BioMart's murine Leptin has been shown to be biologically active in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight.
• Molecular Mass: 16.2 kDa
• Protein length: 147 amino acid
• AA Sequence: MVPIQKVQD DTKTLIKTI VTRINDISH TQSVSAKQR VTGLDFIPG LHPILSLSK MDQTLAVYQ QVLTSLPSQ NVLQIANDL ENLRDLLHL LAFSKSCSL PQTSGLQKP ESLDGVLEA SLYSTEVVA LSRLQGSLQ DILQQLDVS PEC
• Endotoxin: <0.1 ng/ug of the protein.
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Storage: Stored at -20 centigrade.
• Reconstitution: Centrifuge the viral prior to opening. Rconstitute in water to a concentration of 1.0-5.0 mg/ml. Do not vortex. For extened storage, it is recommended to further dilute in a buffer containing a carrier protein(example 0.1% BSA) and store in working aliquots at -20 centigrade to -80 centigrade.

Gene Information

• Gene Name: Lep leptin [ Mus musculus ]
• Official Symbol: Lep
• Synonyms: LEP; leptin; obesity factor; ob; obese
• Gene ID: 16846
• mRNA Refseq: NM_008493
• Protein Refseq: NP_032519
• UniProt ID: P41160
• Chromosome Location: 6 A3.3; 6 12.3 cM
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding