"UCHL5" Related Products

Expand
Expand
Expand

Recombinant Human UCHL5, His-tagged

Cat. No.: UCHL5-1519H
Product Overview: Recombinant human UCHL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-329aa).
Description: Ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves "Lys-48"-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
Molecular Weight: 39.7kDa (349aa), confirmed by MALDI-TOF
Purity: > 90% by SDS - PAGE
Concentration: 0.5mg/ml (determined by Bradford assay)
Biological activity: Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
Activity Assay: 1. Prepare a 100ul of recombinant UCH-L5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC - 96 Well Polystyrene Microplate, black - Fluorescent plate reader
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: UCHL5 ubiquitin carboxyl-terminal hydrolase L5 [ Homo sapiens ]
Official Symbol: UCHL5
Synonyms: UCHL5; UCH37; CGI-70; INO80R; AD-019; UCH-L5; ubiquitin carboxyl-terminal hydrolase L5; ubiquitin carboxyl-terminal hydrolase isozyme L5; OTTHUMP00000033565; OTTHUMP00000033566; OTTHUMP00000033567; OTTHUMP00000033568; OTTHUMP00000033570; OTTHUMP00000033571; OTTHUMP00000033754; INO80 complex subunit R; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5; EC 3.4.19.12
Gene ID: 51377
mRNA Refseq: NM_015984
Protein Refseq: NP_057068
MIM: 610667
UniProt ID: Q9Y5K5
Chromosome Location: 1q32
Function: cysteine-type peptidase activity; endopeptidase inhibitor activity; omega peptidase activity; peptidase activity; proteasome binding; protein binding; ubiquitin thiolesterase activity; ubiquitin-specific protease activity

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.