||Recombinant Human RBP-4 produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa. RBP4 is fused to N-terminus His tag which containes additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16–199. The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.
||Retinol binding protein 4(RBP4) belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||RBP-4 His tag was lyophilized from a concentrated (0.5mg/ml) solution containing PBS pH-7.5.
||It is recommended to reconstitute the lyophilizedRetinol binding protein-4 in sterile deionized water to a working volume of 0.5 mg/mL and let the lyophilized pellet dissolve completely
|Amino acid sequence:
||MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSE RNLL.
||Lyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution Retinol binding protein-4 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.