Active Recombinant Rat Rbp4 protein(Met1-Leu201), His-tagged

• Cat.No.: Rbp4-7427R
• Product Overview: Recombinant Rat RBP4 (NP_037294.1) (Met 1-Leu 201) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-His
• Protein length: Met1-Leu201
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (BC50) is >0.5 μM.
• Molecular Mass: The recombinant rat RBP4 comprises 194 amino acids and predicts a molecular mass of 22.8 kDa. The apparent molecular mass of the rat RBP4 is approximately 24 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Rbp4 retinol binding protein 4, plasma [ Rattus norvegicus ]
• Official Symbol: Rbp4
• Synonyms: RBP4; retinol binding protein 4, plasma; retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein; RBPA
• Gene ID: 25703
• mRNA Refseq: NM_013162
• Protein Refseq: NP_037294