Recombinant Rat vascular endothelial growth factor A, His-tagged

• Cat.No.: Vegfa-677R
• Product Overview: VEGFA, 206-325aa Rat, His tag, E.coli

Specification

• Description: Vascular endothelial growth factor A isoform 3, also known as VEGFA, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. Recombinant Rat VEGFA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Rat
• Tag: His-tag
• Form: Liquid. In Phosphate buffer saline (pH 7.4) containing 50% glycerol
• Molecular Mass: 16.7 kDa (145aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAPTTE GEQKAHEVVK FMDVYQRSYC RPIETLVDIF QEYPDEIEYI FKPSCVPLMR CAGCCNDEAL ECVPTSESNV TMQIMRIKPH QSQHIGEMSF LQHSRCECRP KKDRTKPEKC DKPRR
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164
• Gene ID: 83785
• mRNA Refseq: NM_001110333
• Protein Refseq: NP_001103803
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Endochondral Ossification, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Hemostasis, organism-specific biosystem; Hypertrophy Model, organism-specific biosystem
• Function: cell surface binding; cell surface binding; chemoattractant activity; chemoattractant activity; cytokine activity; cytokine activity; fibronectin binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin bindin