Recombinant Human EPOR & CD131 cell lysate

• Cat.No.: EPOR & CD131-1943HCL
• Product Overview: Human EPOR & CD131 Homodimer derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: EPOR erythropoietin receptor [ Homo sapiens ]; CSF2RB colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) [ Homo sapiens ]
• Official Symbol: EPOR; CD131
• Synonyms: EPO-R; erythropoietin receptor; CD131; IL3RB; IL5RB; SMDP5; CDw131; cytokine receptor common subunit beta; GM-CSF/IL-3/IL-5 receptor common beta-chain; GM-CSF/IL-3/IL-5 receptor common beta subunit; colony-stimulating factor-2 receptor, beta, low-affinity; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3 receptor, beta (high affinity)
• Gene ID: 2057; 1439
• mRNA Refseq: NM_000121; NM_000395
• Protein Refseq: NP_000112; NP_000386
• MIM: 133171
• UniProt ID: P19235; P32927
• Chromosome Location: 19p13.3-p13.2; 22q13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem. Apoptosis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem
• Function: erythropoietin receptor activity; identical protein binding; protein binding; contributes_to interleukin-3 receptor activity; receptor activity