"RAPGEF1" Related Products

Recombinant Human RAPGEF1 293 Cell Lysate

Cat.No.: RAPGEF1-2522HCL
Description: Antigen standard for Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1 [ Homo sapiens ]
Official Symbol: RAPGEF1
Synonyms: RAPGEF1; Rap guanine nucleotide exchange factor (GEF) 1; GRF2, guanine nucleotide releasing factor 2 (specific for crk proto oncogene); rap guanine nucleotide exchange factor 1; C3G; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); GRF2; DKFZp781P1719;
Gene ID: 2889
mRNA Refseq: NM_198679
Protein Refseq: NP_941372
MIM: 600303
UniProt ID: Q13905
Chromosome Location: 9q34.3
Pathway: ARMS-mediated activation, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; Focal Adhesion, organism-specific biosystem;
Function: SH3 domain binding; guanyl-nucleotide exchange factor activity; protein binding;

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