Recombinant Human RAPGEF1 protein, His-tagged

• Cat.No.: RAPGEF1-2180H
• Product Overview: Recombinant Human RAPGEF1 protein(748-1095 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 748-1095 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AASequence: EELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: RAPGEF1 Rap guanine nucleotide exchange factor (GEF) 1 [ Homo sapiens ]
• Official Symbol: RAPGEF1
• Synonyms: RAPGEF1; Rap guanine nucleotide exchange factor (GEF) 1; GRF2, guanine nucleotide releasing factor 2 (specific for crk proto oncogene); rap guanine nucleotide exchange factor 1; C3G; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); GRF2; DKFZp781P1719
• Gene ID: 2889
• mRNA Refseq: NM_198679
• Protein Refseq: NP_941372
• MIM: 600303
• UniProt ID: Q13905