"KCNQ1" Related Products

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Recombinant Human KCNQ1 293 Cell Lysate

Cat.No.: KCNQ1-5020HCL
Description: Antigen standard for potassium voltage-gated channel, KQT-like subfamily, member 1 (KCNQ1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: KCNQ1 potassium voltage-gated channel, KQT-like subfamily, member 1 [ Homo sapiens ]
Official Symbol: KCNQ1
Synonyms: LQT; RWS; WRS; LQT1; SQT2; ATFB1; ATFB3; JLNS1; KCNA8; KCNA9; Kv1.9; Kv7.1; KVLQT1; potassium voltage-gated channel subfamily KQT member 1; slow delayed rectifier channel subunit; voltage-gated potassium channel subunit Kv7.1; kidney and cardiac voltage dependend K+ channel; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
Gene ID: 3784
mRNA Refseq: NM_181798
Protein Refseq: NP_861463
MIM: 607542
UniProt ID: P51787
Chromosome Location: 11p15.5
Pathway: Adrenergic signaling in cardiomyocytes, organism-specific biosystem; Pancreatic secretion, organism-specific biosystem; TNF-alpha/NF-kB Signaling Pathway, organism-specific biosystem
Function: calmodulin binding; outward rectifier potassium channel activity; scaffold protein binding

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