||Recombinant human BNP, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 14 kDa (129 aa).
||BNP (Brain natriuretic peptide) is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. BNP is a 32 amino acid polypeptide and is co-secreted along with a 76 amino acid N-terminal fragment which is biologically inactive. This protein has biological actions including natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion. It is thought to play a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MHPLGSPGSA SDLETSGLQE QRNHLQGKLS ELQVEQTSLE PLQESPRPTG VWKSREVATE GIRGHRKMVL YTLRAPRSPK MVQGSGCFGR KMDRISSSSG LGCKVLRRH
||Liquid in Phosphate-Buffered Saline (pH 7.4), 40%glycerol, 2mM DTT, 0.1mM PMSF, 1mM EDTA
||14 kDa (129 aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
||< 1.0 eu per 1 μg of protein (determined by lal>
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.