| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
27-102 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Terminal pro-brain natriuretic peptide (NT-proBNP) is a prohormone with a 76 amino acid N-terminal inactive protein that is cleaved from the molecule to release brain natriuretic peptide (BNP)1. NT-proBNP may affect cardio-renal homeostasis and it can oduction of cGMP although its potency is very low compared to brain natriuretic peptide 32. C Reactive Protein (CRP) can interact with NT-proBNP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human NT-proBNP and recombinant human CRP. Briefly, NT-proBNP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CRP-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NT-proBNP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human NT-proBNP and recombinant human CRP was shown, the EC50 for this effect is 4.2 ug/mL. |
| Molecular Mass : |
10.1kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
