| Description : |
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. |
| Source : |
Baculovirus |
| Species : |
Human |
| Tag : |
His |
| Form : |
20mM Tris-Cl (pH7.9), 20% Glycerol, 100mM NaCl, 1mM DTT and 0.5mM EDTA |
| Bio-activity : |
DNA Topoisomerase I catalyzes the latering the topology of DNA during transcription and replication. Recombinant Topo I protein is ideal for the studies of DNA topology and other related function assays. |
| AA Sequence : |
MHHHHHHGRRASVEFSGDHLHNDSQ IEADFRLNDSHKHKDKHKDREHRHK EHKKEKDREKSKHSNSEHKDSEKK HKEKEKTKHKDGSSEKHKDKHKDRD KEKRKEEKVRASGDAKIKKEKENGF SSPPQIKDEPEDDGYFVPPKEDIK PLKRPRDEDDADYKPKKIKTEDTKK EKKRKLEEEEDGKLKKPKNKDKDKK VPEPDNKKKKPKKEEEQKWKWWE EERYPEGIKWKFLEHKGPVFAPPYE PLPENVKFYYDGKVMKLSPKAEEVA TFFAKMLDHEYTTKEIFRKNFFKD WRKEMTNEEKNIITNLSKCDFTQMS QYFKAQTEARKQMSKEEKLKIKEEN EKLLKEYGFCIMDNHKERIANFK IEPPGLFRGRGNHPKMGMLKRRIMP EDIIINCSKDAKVPSPPPGHKWKEV RHDNKVTWLVSWTENIQGSIKYIM LNPSSRIKGEKDWQKYETARRLKKC VDKIRNQYREDWKSKEMKVRQRAVA LYFIDKLALRAGNEKEEGETADT VGCCSLRVEHINLHPELDGQEYVVE FDFLGKDSIRYYNKVPVEKRVFKNL QLFMENKQPEDDLFDRLNTGILNK HLQDLMEGLTAKVFRTYNASITLQQ QLKELTAPDENIPAKILSYNRANRA VAILCNHQRAPPKTFEKSMMNLQ TKIDAKKEQLADARRDLKSAKADAK VMKDAKTKKVVESKKKAVQRLEEQL MKLEVQATDREENKQIALGTSKLN YLDPRITVAWCKKWGVPIEKIYNKT QREKFAWAIDMADEDYEF |
| Purity : |
≥90%, as determined by SDS-PAGE |
| Usage : |
FOR RESEARCH ONLY |
| Storage : |
The protein sample can be stored under sterile conditions at 2- 8 centigrade for one month or at -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
