Recombinant Mouse Top1 Protein, His-tagged

• Cat.No.: Top1-465M
• Product Overview: Recombinant Mouse Top1 Protein (Met1-Ser252) with N-His tag was expressed in E. coli.

Specification

• Description: Enables DNA topoisomerase type I (single strand cut, ATP-independent) activity. Acts upstream of or within DNA replication; DNA topological change; and embryonic cleavage. Located in cytoplasm; nucleus; and perikaryon. Is expressed in brain; cerebral cortex ventricular layer; cortical plate; and telencephalon. Orthologous to human TOP1 (DNA topoisomerase I).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.1 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Met1-Ser252
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Top1 topoisomerase (DNA) I [ Mus musculus (house mouse) ]
• Official Symbol: Top1
• Synonyms: TOP1; topoisomerase (DNA) I; DNA topoisomerase 1; DNA topoisomerase I; Top-1; AI467334; D130064I21Rik
• Gene ID: 21969
• mRNA Refseq: NM_009408
• Protein Refseq: NP_033434